FLOW CYTOMETRY

1. FOR CANCER PATIENTS, RERPODUCTIVITY FAILURES, TRANSPLANTATIONS, DIABETES,
IMMUNOPHENOTYPE), CYTOTOXICITY TEST OF NK CELLS
TESTING
(ANTIBODIES):
CD45,CD2, CD3, CD4, CD8, CD14, CD19, CD16+56, CD 25, HLA-DR,TCRαβ, TCRγδ, ΝΚ cells

2. CHRONIC LYMPHOPROLIFERATIVE DISORDERS – LYMPHOMAS.
CHECK OF SURFACE ANTIBODIES
TESTING ,
(SURFACE ANTIBODIES):
CD19, CD5, CD20, CD38, CD45, CD23, CD43, CD11c, FMC7, CD79b, CD79a.
CD1a, TCR, CD30, CD56.
KAPPA, LAMBDA, IgM, IgD, IgG, IgA
CD103, CD22, CD25, CD138

CYTOPLASMIC ANTIBODIES):
ZAP70, KAPPA, LAMBDA, , IgM, IgD, IgG, IgA, CD3

(QUANTITATION):
CD20, CD38

3. ACUTE LYMPHOBLASTIC LEUKEMIA
TESTING
(SURFACE ANTIBODIES):
CD45, CD1a, CD2, CD3, CD5, CD7, CD4, CD8, CD19, CD20,
CD22,CD79a, CD10, IgM, IgD, IgG, IgA, HLA-DR,

(CYTOPLASMIC ANTIBODIES):
TdT, IgM, CD3.

4. ACUTE MYELOBLASTIC LEUKEMIA)
TESTING
(SURFACE ANTIBODIES):
CD45, CD2, CD13, CD14, CD15, CD19, CD33, CD34, CD56, CD61,
CD64, CD117, HLA-DR, CD 36, CD71, GLYCOPHORIN A, CD41, CD61.

(CYTOPLASMIC ANTIBODIES):
MPO, LYSOSYME.

5. (PNH)
TESTING
(SURFACE ANTIBODIES) :
CD55, CD59(IN THE NEUTROPHILS AND THE RED BLOOD CELLS).

6. (ΙΜΜUNOPHENOTYPE FOR DIAGNOSIS IN IMMUNODEFICIENCIES) SCID
(SURFACE ANTIBODIES): CD3, CD4, CD8, CD16, CD56, CD19, CD20, CD40L, HLA-DR, TCRαβ, TCRγδ, CD45RO, CD45RA, CD62L,
CD25, CD69, CD27, CD28, CD80, CD86.

(CYTOPLASMIC CYTOKINES):
IL-2, IL-4, IL-5, IL-6, IL-10, INF-γ, TNFa.

(PHAGOCYTOCIS)

7. CHECKING OF HIV(+) PATIENTS
(SURFACE ANTIBODIES):
CD45, CD14, CD3, CD4, CD8, CD19, CD16+56, CD45RA, CD45RO, CD38.

(QUANTITATION ):
CD38.

8. DNA ANALYSIS
CELL DETECTION IN EACH PHASE
1) RELAXATION (G0/G1
2) SYNTHESIS DNA (S)
3) MITOSIS (G2/M)
DNA INDEX

9. (APOPTOSIS DETECTION)
DETECTION OF CHANGES IN THE CELLULAR MEMBRANE:
ΑΝΝΕΧΙΝ V, PROPIDIUM IODIDE, APO-1, CD95.
ANALYSIS OF ENDOCELLULAR ORGANELLES
BCL-2

CASPACES DETECTION

DETECTION OF DNA INDEX

10. MEASUREMENT OF CD34+ (STEM CELLS)
CD45/CD34 WITH PROCOUNT KIT

11. MEASUREMENT OF PROTEINS, CYTOKINES BY THE USE OF BEADS
TH1/TH2 KIT (IL-2, IL-4, IL-6, IL-10, TNF-a, IFN-g)
INFLAMMATION KIT (IL-8, IL-1b, IL-6, IL-10, TNF, IL-12p70)
ANGIOGENESIS KIT (IL-8, BFGF, VEGF, ANGIGENIN, TNF)
ANAPHYLATOXIN KIT (C4a, C3a, C5a)
CHEMOKINE KIT (IL-8, RANTES, MIG, MCP-1, IP-10
ΜΕΤΡΗΣΗ ΚΥΤΤΑΡΟΚΙΝΩΝ ΣΕ ΔΑΚΡΥΙΚΟ ΥΓΡΟ
BRONCHOALVEOLAR LIQUID (BAL)
CD15 FITC /CD23PE /CD45 PerCP
CD3+, CD4+,CD8+,CD25+, CD3+HLA-DR+
CBA Th1/Th2
CBA Inflammation
CBA Chemokine
FERTILITY: CΒΑ:BDCΒΑ ΤΗ1/ΤΗ2

12. DNA FRAGMENTATION OF SPERM

13. DETECTION OF DEGRANULATION OF BASOPHILS

14. (PLATELETS FUNCTION)
PAC-1, CD41, CD42a, CD42b, CD61, CD62P